| Department |
1.Taiwan Provincial Research Institute for Animal Health, Tansui, Taipei, Taiwan, R.O.C.
2.Dept. Veterinary Medicine, College of Agriculture, National Taiwan· University, Taipei, Taiwan, R.O.C.
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| Summary |
Ten ducks were highly immunized with purified swine lgG. The anti-swine lgG antiserum, 350ml from the ten im-munized ducks, was salted out at 40% saturation in ammonium sulfate and then chromatographed through Sephacryl S-300 column. The 7S and SS serum globulins were collected and absorbed with Sepharose CL4B conjugated by swine lgG. The duck ant,-swine IgG antibody absorbed in the affinity column was desorbed by O. IM glycine buffer, pH 2.5. Only one precipita-tion line of the desorbed duck immunoglobulin was observed when tested in im-munoelectrophoresis against rabbit anti-duck whole serum antiserum. This would suggest that the immunoglobulin isolated in this manner is the duck IgG according to the location of the precipitation line in the immunoelectrophoresis analysis. Anti-duck JgG antiserum was raised by injecting a rabbit with the isolated duck JgG. Only one precipitation line of the anti-duck IgG antiserum when tested in immuno-electrophoresis against duck whole serum.
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