AHRI report No.26

Preparation of Hog Cholera Virus Monoclonal Antibody for Diagnostic Reagent

1.     Taiwan Provincial Research Institute for Animal Health. 
2.     Pig Research Institute, Taiwan. 

Hog cholera (HC) virus A76 strain propagated in PK-15 Cultured cells was purified by sucrose density gradient centrifugation and ultracentrifugation. One hybr-idoma designated as A76 115 was obtained from spleen cells of Balb/c mice immun-ized with A76 strain of purified HC virus fused with NS- I myeloma cells. It was capable to secrete monoclonal antibodies against HC viral antigen detected by indir-ect immunofJuorescent techniques (IFA). Monoclonal antibody secreted by A76 115 hybridoma was applied in IFA to detect ALD strain of HC viral antigen in the infected tonsi11ar tissue prepared by frozen sections and to detect the HC viral antigens propagated in the rabbit testicle and PK-15 cells. The fluorescence-stained particles in the nuclei of cultured cells were noted. This phenomenon is still inexpl-icable. The immunoperoxidase monolayer assay (IPMA) performed on cultured cells was capable of detecting HC viral antigens in the cytoplasm; however, it is not yet possible to differentiate between the A76 and LPC strains of viral antigens with A76 115 monoclonal antibody. 

hyhidoma; Hog Cholera Vaccine;