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Update Date: [2022-09-20]

Publication Studies on the Production of Pseudorabies Vaccine for Swine:Investigation on the Pertinent Cell Line Cultures for Replication of the Isolated Viruses of N3 and T3 Strain

Reportno.

AHRI report No.13

Topic

Studies on the Production of Pseudorabies Vaccine for Swine:Investigation on the Pertinent Cell Line Cultures for Replication of the Isolated Viruses of N3 and T3 Strain 

Department 1.Taiwan Provincial Research Institute for Animal Health
2.Joint Commission on Rural Reconstruction
Author K. F. Lin., T. C. Chiu, S. S. Cheng. ;Y. H. Liu. 
Summary

Two isolates, N3 and T 3 were cultivated in various cell cultures, and the infective titers of extra-cellular virus, intra-cellular virus were determined in every other period of 5 days. From those growth curves of the isolated viruses, We have known the pertinent cell line cultures are RK-13 and ESK. Virus titer reached 10,.0-.., TC!D50/0. 1ml at 12 to 24 hours postinoculating in RK-13 cell line and 107,0-..s TCIDso/0. l ml at 24 to 48 hours postinoculating in ESK cell line. Virus titer reached around l06·a-c·7TCID50/0. lml at 24 to 96 hours postinoculating in PK-15, AuBEK, BK, MK, and KB cell lines. 
The pathogenicity of the two isolates were weakened for rabbit after 20 subpassages in RK-13 and ESK. 

Keyword

Pseudorabies Vxcine N3 ; T3 ; pxtinccu]ating

 


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