Summary |
A Hight rerformance liqpid c]iromatographic methcd for simulneous determination of thiamphenkol (W) and chloranphenicol (G4 ) in porcine and poultry fls has ka developel. These feds were extractM with atetonitri]e then purifisi with SEP PAK alumina N rtridge. With TSK-GEL ODS-l2OTcolumn and Si,unmary &xtonitile:0.05 % phosphoric (18:82) as mobile phase, a gocd sereration and linear calibration carves of W and CM could Ire obtainal by keing deteel at 230 nra svthzhing to 280nn. Reookeries of W and CM in rorcire feds were %.9 % and 73.6 %, respertively, Recokeries of Wand CM in poultry feds were 76.2 % and 65.8 %, resertive1y,
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