| Summary |
A reversed-phase high performance liquid chromatographic ( HPLC ) method was developed for simultaneously analysing amprolium and ethopabate in chicken feeds. The drugs were extracted from samples with methanol and were filtered. The methanolic filtrate was cleaned up by solid-phase extraction. HPLC was done on the Cosmosil 5 C8-MS column with methanol-water ( 40 : 60) , containing octanesulfonic acid, triethylamine and glacial acetic acid, as mobile phase. The analytes were detected at 270 nm. Average recoveries for amprolium-fortified samples at 2.5 and 1.25 ppm were 92.3%( coefficient of variation, CV, 1.4% ) and 95.3% (CV, 7.1% ), respectively. Average recoveries for the ethopabate-fortified samples at 0.4 and 0.2 ppm were 90.0 % ( CV, 7.1 % ) and 83.9 % ( CV, 4.2% ) respectively. The levels of detection limit for amprolium and ethopabate were 0.025 ppm and 0.015ppm, respectively.
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| Keyword |
Amprolium, Ethopabate, Simultaneous determination, Chicken feeds, High performance liquid chromatography ( HPLC )
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