| Summary |
For the development of a rapid diagnostic technique of Newcastle disease, two
primer pairs were designed and used in the reverse transcription polymerase chain reaction ( RT-PCR ) for the detection of Newcastle disease virus ( NDV ). Amplified cDNA of NDV was confirmed by electrophoresis and staining of ethidium bromide. The technique was further applied to the diagnosis of suspected Newcastle cases occurring in Prefectures of Tainan, Chuanghwa, Taoyuang, Mialee and Hsingchu during January 1997 to August 1998. Specific PCR products could be amplified and confirmed in all cases of Newcastle disease which were diagnosed by routine virus isolation and identification. The RT-PCR technique developed could be used first and before any other type of examination for the rapid, tentative diagnosis of Newcastle disease.
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