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Update Date: [2022-10-06]

Publication Electrophoresis Patterns of the Outer Membrane Protein in Riemerella anatipestifer


Reportno.

AHRI report No.36

Topic

Electrophoresis Patterns of the Outer Membrane Protein in Riemerella anatipestifer

Department

1.    National Institute for Animal Health, Council of Agriculture, Executive Yuan
2.    Department of Veterinary Medicine, National Taiwan University

Author Lin, W. H.1*, S. L.Chen1, J. F. Su1, T. L. Hsu1, C. F. Chang2, S. Y. Lin1 
Summary

These experiments were designed to investigate the biological characteristics of the outer membrane proteins of Riemerella anatipestifer. The outer membrane proteins were extracted from 25 strains of Riemerella anatipestifer, which were isolated from ducks and geese with infectious serositis. Bacterial cells were sonicated and extracted with sarcosine. The extracted outer membrane proteins were analyzed by SDS-PAGE. The 25 strains of bacteria could be divided into two groups based on electrophoresis patterns of the outer membrane proteins. Group one including 20 strains showed identical patterns on SDS-PAGE, except nine of the 20 strains, an extra band with 55 kDa was 
observed. Group 2 including 5 strains showed different electrophoresis patterns. 

Keyword

 Riemerella anatipestifer, Outer membrane proteins, Bacterial cells