| Summary |
ased on the studies of viral characterization and amino acid sequences, the antigenicity of hog cholera virus between LPC vaccine strain and field isolates was analyzed. Thirty-six field isolates chosen from 7 different restriction patterns were tested by exaltation of Newcastle disease (END) method and E 一 interference test. The results indicated that 34 isolates belonged to the highly virulent strains and 2 isolates belonged to the less-virulent strains. LPC vaccine strain could be distinguished from ALD strain by determination of plaque sizes in indirect immuno-peroxidase plaque staining test (IIPS). however, the field isolates always exhibited different plaque sizes. By comparing the E0, E1 and E2 glycoprotein gene sequences between LPC and ALD strain, the similarity of amino acids was 94.7 %, however, there was a highly variable region in the B domain of the E2 gene. In this highly variable region, six in 10 amino acids were different between sequences 471 to 480.
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