| Summary |
The phenol-extracted lipopolysaccharides (LPS) gave a positive reaction both in Limulus amboebocyte lysate (LAL) test and dermal Shwartzman reaction, but not in hemolysis test. The SOS-PAGE pattern of extracted LPS revealed by silver stain showed a ladder form and was considered as a smooth type of LPS pattern. The LO so of LPS was 8.9 mg in mice when intraperitonea11y injected with 0-gaJactosa-mine. It was also found that the soluble antigens of HP contained large amounts of LPS antigen. Two immunoprecipitation lines of LPS which were serotype-specific were observed when antiserum against homologus serotype of HP was used in double immunodiffusion test. Intratracheal inoculation of LPS into guinea pigs induced con-gestion and haemorrhage of lung. Histopathologically, extensive bronchopneumonia and aggregation of spindle, pyknotic cells were observed. The lesions induced by LPS in guinea pigs were similar to those induced by live HP inoculation. The immunogen-ecity of LPS was tested by using mouse protection test. The experimental data indicateed that LPS antigen gave a good protective rate.
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