Previous serological investigation revealed that Taiwan was still free of rinderpest and African swine fever. Continuous nationwide surveillances on cattle, goats and pigs are essential to confirm the free status. Sera for rinderpest and African swine fever antibody detection will be sampled by local disease control centers and submitted to Animal Health Research Institute for detection of antibodies to specific pathogen by enzyme-linked immunosorbent assay (ELISA). In addition, in order to establish a reliable method to monitor the distribution of bluetongue in the country, comparison of the serological for bluetongue antibody detection was conducted as well. Pathogenecity of bluetongue isolates to sheep was also studies in our work.