Reporton.

AHRI report No.47

Topic

Development of Passive Protection Antibodies against Riemerella anatipestifer Infection Isolated from the Egg Yolk of Vaccinated Chicken/Duck

Department

Animal Health Research Institute, Council of Agriculture, Executive Yuan

Author

CF Yu, TS Huang, CC Huang

Summary

In order to develop therapeutic antibodies againstRiemerella anatipestifer (RA) infection, chicken and duck were hyper-immunized with RA serotype 2 bacterin, and their egg yolks were collected and processed to obtain chicken and duck water soluble fractions (CWSF and DWSF) using the water dilution method. The antibody titers against RA from the serum samples and WSFs were determined by indirect enzyme-linked immunosorbent assay (ELISA) and tube agglutination test. The IgYs in WSFs were further confirmed by Western blot analysis. The results showed that both CWSF and DWSF could provide effective passive protection against homologous serotype RA challenge in Peking duck. CWSF also showed a protective passive protection against homologous challenge in a specific-pathogen free Muscovy duck test model.

Keyword

Riemerella anatipestifer infection, egg yolk antibody, water soluble fraction, water dilution method, potency test.