Severe reaction was seen by subcutaneous injection with concentrated and heat-inactivated PrV antigen into lower eyelid of swine. This reaction was verified as d-elayedtype hypersensitive reaction by histopathological changes. Nonexposed control pigs did not react to the viral and cellular antigens at all. The correlation among serum neutralization, microimmunodiffusion, and skin tests was analysed. The results indicated that the sensitivities of the latter two tests were pretty high and similar to each other. The antigens obtained by treating the infected cells with proteolytic enzyme were suitable for microimmunodiffusion test.