| Summary |
A polymerase chain reaction ( PCR ) was applied to diagnosis the pathogen of Chlamydia psittaci. The annealing temperature of primer was adjusted to be 55 °C in PCR protocol. The sensitivity of PCR was detectable the chlamydia! doses of 0.001 LD50 which was reciprocal in chicken embryos. For the clinical trial, the relative sensitivity was 95 %, specificity was 86 % and the agreement was 88.6 % among 256 samples. According to the results, we performance suggested that the PCR was a validated assay for diagnosis of chlamydia! infection.
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