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The purpose of this study is to develop a trivalent inactivated bacterin against Riemerella anatipestifer (RA) infection. At first, we have finished the collection, selection and identification of 21 serotypes of RA strains. The hyperimmunized sera against 21 serotypes of strains were also produced by using the rabbit models and they will be used for fast identification of field strains in the future. The strains including serotypes 1, 2 and 6 were preliminarily selected to develop a trivalent bacterin.
We have also established various culture methods, and performed the comparison of different results to determine the best conditions for the induction of immune efficacy. The results showed that there is no significantly difference on potency tests of the trivalent bacterin prepared from different culture time or media. The 1/2 and 1/10 dosages could not provide enough protection against challenge. Several potency tests have shown that the most protection indexes of the bacterin have reached the national standards of animal drug inspection.
The development of an ELISA kit for detecting duck serum antibodies against RA antigens has shown sufficient sensitivity and susceptibility.
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