| Reportno. |
AHRI report No.42 |
| Topic |
Expression of the Recombinant ORF 2 Protein of Porcine Circovirus Type 2 in Eukaryotic System |
| Department |
1Animal Health Research Institute, Council of Agriculture, Executive Yuan
2Graduate Institute of Veterinary Medicine , National Taiwan University |
| Author |
Chun Wang*1.2, Jui-Yu Chiu1.2, Tien-Shine Huang1, Ming-Hwa Jong1, Parn-Hwa Zhao1 and Ing -Cherng Guo
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| Summary |
Porcine circovirus type 2 represents the causative agent of a new porcine disease which spread all over the world during the past several years. In this study, viral DNA of a Taiwanese PCV2 strain was extracted and its open reading frame 2 (ORF2) gene was amplified by PCR. The PCR product was purified and then cloned into the pCI-neo vectors. The recombinant vector was transfected into PK-15 cells. The recombinant protein was expressed successfully in PK-15 cells, which could be detected by using indirect immunofluorescence assay and Western blotting analysis.
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| Keyword |
Porcine circovirus type 2; Indirect immunofluorescense assay; SDS-PAGE; Western blotting
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