| Summary |
Monoclonal antibodies(Mabs). have been widely used in biological researches, medical diagnoses and therapies. The production of Mab from ascites by mouse can make a high yield；however, the use of animals for large-scale production of Mab is restricted for the animal welfare concerns. In the current trend, many in vitro alternatives have been developed for Mab production. In this study the hybridoma cell culture was performed in a 500 mL spinner basket containing 6 g non-woven polyester fabric (NWPF) microcarrier. Cells for inoculum were prepared at density 1x105 cell.mL-1 of 430 mL culture medium. Cells were growing in batch culture until day 9 when the perfusion process was initiated. After 5 more days, culture was discontinued. During the 14 days culture period, four liters supernatant was harvested in total. ELISA was applied to evaluate the activity of secreting monoclonal antibody (Mab) from culture . According to the result, the Mab activity from spinner basket culture was 2-4 times higher than T-flask culture in control, and the Mab yield of operation was equivalent to 160 T175 flasks. Application of NWPF material could be a promising alternative to Mab production while optimal culture condition was established.
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