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Update Date: [2022-10-05]

Research Information Surveillance and monitoring of transmissible spongiform encephalopathy and rabies and serological survey of dog antibody against rabies with immunization


Reportno.

AHRI report No.33

Topic

Application of the Immunoperoxidase Staining Techniques in the Diagnosis of Avian Viral Infection of Central Nervous System 

Department

Taiwan Animal Health Research Institute. 

Author S. H Lee,* M. H Jong, Y. S. Lu, D. F. Lin, M. C. Cheng and S. G Yang
Summary

The immunoperoxidase staining techinques (direct and indirect) were applied 
to detect AI (avian influenza), ND (Newcastle disease) and AE (avian encephalomyelitis) virus antigens in formalin fixed , paraffin-embedded section of experimentally and naturally infected chickens. The immunoperoxidase staining technique (IP) was compared with histopathological examination and virus isolation for detection of AI, ND and AE virus in tissues of infected chickens. It is concluded that the IP techinque is a good and specific supplementary diagnosis method for viral infection of avian diseases. 

Keyword

lmmunoperoxidase staining , Newcastle disease , Avian encephalitis , Avian infuenza

 


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