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Update Date: [2022-09-23]

Publication STUDIES ON BRUCELLA SUIS IN ARTIFICIALLY INFECTED PIGS AND SEROLOGICAL INVESTIGATION ON THE DISEASE IN TAIWAN

Reportno.

AHRI report No.18

Topic

STUDIES ON BRUCELLA SUIS IN ARTIFICIALLY INFECTED PIGS AND SEROLOGICAL INVESTIGATION ON THE DISEASE IN TAIWAN

Department

1.     Taiwan Provincial Research Institute for Animal Health 
2.     Yun-Lin Livestock Disease Control Center 
3.     Taipei Hsien Livestock Disease Control Center 
4.     Department of Forestry and Agriculture, Taiwan Provincial Government. 

Author

D. F. LIN1 , Y. S. LU 1 Y. L. LEE 1 • K. S. TSAI2 ,G. Z. CHEN', C.H. WANG1 , and I.S. TSAI4 

Summary

Antibody response in Brucella suis (Strain #686) inoculated pigs was deter-mined by means of agglutination and complement-fixation tests. The agglutination antibody was firstly detected at one week postinoculation, peaked at 5-9 weeks P.1. (with level of I :80-1: 160) and lasted until 20 weeks P.1. or even longer. The CF antibody can not be detected until 3 weeks P.1. The level reached peak at 7 weeks P.I., and also maintained up to 20 weeks P.I. or longer. 
Testis swelling was observed in the inoculated pig 7 week P.1., and markable atrophy noted 19 weeks P.1. The bacteria could be recovered from the testis when the pig necropsied at 22 P.1. During the period 1979-1980, 8319 serum samples were collected from pigs in 13 counties, and subjected to plate agglutination test against Brucella suis. Six hundred and seventy-four out of the 8319 samples were positive (I :80) in the plate agglutination test, and 615 out of the 674 were subjected to tube agglutination (TA) and CF tests. Ninety-one (14.8%) of them had the titer I :30, and 34 (5.5%) of them 1 :40 (100 IU) in the TA test, while all of them were negative in CF test. 
The data suggested that sera with level of antibody above I :40 (100 IU) in tube agglutination test and positive (I :5) in CF test may be considered as positive in the disease. 

Keyword

Brucella suis ; CF test

 


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