Author |
D. F. LIN1 , Y. S. LU 1 Y. L. LEE 1 • K. S. TSAI2 ,G. Z. CHEN', C.H. WANG1 , and I.S. TSAI4
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Summary |
Antibody response in Brucella suis (Strain #686) inoculated pigs was deter-mined by means of agglutination and complement-fixation tests. The agglutination antibody was firstly detected at one week postinoculation, peaked at 5-9 weeks P.1. (with level of I :80-1: 160) and lasted until 20 weeks P.1. or even longer. The CF antibody can not be detected until 3 weeks P.1. The level reached peak at 7 weeks P.I., and also maintained up to 20 weeks P.I. or longer.
Testis swelling was observed in the inoculated pig 7 week P.1., and markable atrophy noted 19 weeks P.1. The bacteria could be recovered from the testis when the pig necropsied at 22 P.1. During the period 1979-1980, 8319 serum samples were collected from pigs in 13 counties, and subjected to plate agglutination test against Brucella suis. Six hundred and seventy-four out of the 8319 samples were positive (I :80) in the plate agglutination test, and 615 out of the 674 were subjected to tube agglutination (TA) and CF tests. Ninety-one (14.8%) of them had the titer I :30, and 34 (5.5%) of them 1 :40 (100 IU) in the TA test, while all of them were negative in CF test.
The data suggested that sera with level of antibody above I :40 (100 IU) in tube agglutination test and positive (I :5) in CF test may be considered as positive in the disease.
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