| Summary |
Bacterial cell suspensions of Bordetella bronchiseptica (Bb), Pasteurella multocida (Pm) and Actinobacillus pleuropneumoniae (Ap) were separately collected and inactivated by adjusting their concentrations. Afterward, the poly-valent bacterin was prepared via aluminum hydroxide gel using combined cell suspensions. The bacterin was used to immunize the pregnant sows first with 3 mL IM for basic vaccination dose at 4 -5 weeks before parturition and boosted with 5 mL two weeks later. In laboratory, agar gel immunodiffusion tests were performed for the colostral antibody titers obtained from sows immunized with poly-valent bacterin after parturition. The antibody titers for the three immunized sows to Pm type A, type D, Ap type 1 and type 5 were 1 : 32, 1 : 16, 1 : 16, 1 : 8, 1 : 4, 1 : 4 : 1 : 16, 1 : 8, 1 : 8 and 1 : 16, 1 : 4, 1 : 8, respectively. However, the precipitation line could neither be detected in the colostrum and serum of sow of the control group nor in sera of the immunized group by the tests. In two field tests, the antibody titers of sows against Bb before vaccination were notably low and increased remarkably after immunization. The titers after vaccination were 1 : 320-1 : 5,120 and 1 : 640 -1 : 20,480 and followed normal distribution pattern. On the other hand, the titers of the 4-day-old suckling piglets were similar to those in the immunized sows. At 8 weeks old, the detected piglet titers were greater than 1 : 40 except for one sample. However, they gradually decreased at 12 and 16 weeks old. Therefore, we can conclude on the basis of the experimental results that poly-valent bacterin could develop effective immune responses for sows, in addition, maternal antibody titers of the suckling piglets were high, furthermore the booster vaccination for piglets after 8 weeks old would be suitable for swine atrophic rhinitis pasteurellosis and actinobacillus pleuropneumonia control. [ * Chen C, Lu CC, Lai JS, KO HJ, Chan IP. Production and field trial of poly-valent bacterin for swine atrophic rhinitis pastenrellosis and actinobacillus pleuropneumonia control.
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| Keyword |
Bordetella bronchiseptica, Pasteurella multocida,Actinobacillus pleuropneumoniae, Poly-valent bacterin, Pleuropneumonia
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