| Summary |
A multiplex polymerase chain reaction (mPCR) assay, using two sets of primers designed according to the sequences of invA and IS200 genes, was developed for the identification of Salmonella. The mPCR assay was evaluated by using 12 seed strains and 251 isolated strains, which were attributed to more than 23 different serovars of group A, B, C1, C2, D1, D2, E1 and E2. All strain tested, except 2 possessed z4 complex (H antigen) strains, were positive in the mPCR assay. The mPCR assay was compared with the traditional culture and biological characteristics to determine the specificity and sensitivity of the test. Using a total of 450 swine fecal samples collected from slaughterhouse during 9 months, 49 samples were identified to be positive in both the mPCR assay and the traditional culture and biological characteristics. In addition, 50 duck fecal samples collected from animal house of duck were also tested, and all were found to be negative in both assays. Thus, the mPCR assay developed can be practically used as an auxiliary step for identifying Salmonella.
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