| Summary |
The polymerase chain reaction (PCR) with five (1 5th) oligonucleotieds primers based on the sequence of hemolysin I and two (6, 7th) primers based on the sequence of hemolysin 2 was used to amplified specific DNA fragment for detection and indentification of Actinobacillus pleuropneumoniae (AP). Serotype 1, 4, 5, 6, 7 and 9 of AP had a positive reaction with first primers. Serotype 1, 5 and 9 had a postitve reaction with 2, 3th primers, but only Serotype 1 and 9 showed positive with 4, 5th primers. By using 6, 7th primers, all serotype l 9 were positive. There was no cross reaction among E. coli, P. multocida, S. chorelBsuis, B. bronchiseptica observed with all 7 primers.
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