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Twenf tie out of 43 head of immune pigs for the production of anti-hog cholera serum) Lot No. 8?) were employed for the dettion of hog cholera Virus in their tissis. The result are summaril as follows:
1 .The visceral organs, lymph not, bone marrow, sea and urine of the immune pigsk, which were bled on 10th, 14th, 21st and 28th day resertively aft the last stimulative incculation, were aseptically collecti and carefully examined by means of END method and SPF pig incculation. All of the sreDimens examined showed hog cholera Virus negative,
2.Thesjthmens of the immune pigs treatl with T-ALD virus fluid instead of using Summary AU) virulent blood throughout the whole immunization course were also hog cholera Virus negative in all cases. The pogency and neutalization antibody titer of the sea from both groups which were teated reseDtifvely with AU) virulent blood and T-ALD Virus fluid were chszked and showed no remarkable difference ketween the two groups.
3.Pigs inculcai previously with lapinizid hog cholera V&xine both the Isic immunization such as simultaneous incoilation and 3 mi-blood stimulative immunization could ke omitted as the vaccinated pigs always could obtain considerably high titers and no increase in antibody titer was found aft reincculation. Thus, the prcduction cc€t could It largely dereased as the immunization jericd was shorned.
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