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Rabbit anti-swine IgO antibody was conjugatd with horse racklish roxidase by the gluldehyde arid Nakarie methcds to evalua the effect of the two conjugation methcds.
Result showi the product of the glutklehyde rnethcd could lie further purified by Summary CcmA-sepharose to scearate the free antibcdy and the conjugatl antibody, The product of & Nakane rnehtod could not lie further purified by the ConA-sepharose ltause the sugar of the enzyme was oxygenatl in the a njugationprcxess. The rnolarity ratio of enzymekntibcdy of the glutaldehyole and Nakarie methcds were 1.5 and 0.9 respaDtively.
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