| Summary |
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of the residue ofEnrofloxacin (ERFX) and Ofloxacin (OFLX) in poultry tissues was developed. Samples were extracted and purified with 0.01N Hydrochloric acid-Acetonitrile (7 : 3 v / v) and SEP-PAK C18, concentrated by nitrogen to dryness, and then analysed by HPLC. The HPLC conditions were established on a Cosmosil 5C18-MS column with pH 3.0 Phosphate buffer / Acetonitile (80 : 20) as mobile phase and detected by fluroresence detector Ex 295 nm , Em 445 nm. Recovery studies were performed at 0.05 ppm levels in each of the three tissues. Average recoveries were 82.3 ~ 92.6 %. The detection limit of residual drugs was 0.005 ppm in samples. Total 112 samples of Poultry tissue from grocery market located in seven counties of Taiwan were surveyed by random for the residues of Enrofloxacin and Ofloxacin with the developed method. The result indicated postive rates of ERFX and OFLX among the samples were 19.6 % (0.095 ~0.005 ppm) and 2.7 % (0.099~0.017 ppm) respectively.
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