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Reporton.
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AHRI report No.46
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Topic
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Development of ELISA for Detecting the Antigens of Foot-and-Mouth Disease
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Department
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1Animal Health Research Institute, Council of Agriculture, Executive Yuan
2Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University
3Zoonoses Research Center, School of Veterinary Medicine, National Taiwan University
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Author
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Tsu-Han Chen1,2*, Yeou-Liang Lin1, Fan Lee1,2, Sue Min Huang1,2,
Chia-Ni Shih1, Chin-Cheng Huang1, Hsiang-Jung Tsai2,3
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Summary
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Sandwich enzyme-linked immunosorbent assay (Sandwich ELISA) was developed to detect antigens of foot-and-mouth disease virus (FMDV). Anti-FMDV monoclonal and polyclonal antibodies were derived from either recombinant FMDV proteins or whole inactivated FMDV. The antibodies were labeled either with horseradish peroxidase or biotin and were incorporated into an ELISA test platform for antigen detection. Our preliminary results indicated that antibodies were able to capture the antigens in samples with FMDV and showed a clear distinction, and without cross reaction between monoclonal and polyclonal antibody sets. The specificity of the ELISA test for seven serotypes of inactivated FMDV was high. The sensitivities of RT-PCR, commercial available chromatographic strips and our ELISA were 10-6, 10-3 and 10-3, respectively. The results indicated that ELISA screening could detect FMDV antigens in the test samples and did not cross-react with swine vesicular disease virus (SVDV) antigens.
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Keyword
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Foot-and-mouth disease virus (FMDV), Swine vesicular disease virus (SVDV), Enzyme-linked immunosorbent assay (ELISA), Chromatographic strip, Antigen detection, Reverse transcription polymerase chain reaction (RT-PCR
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