| Summary |
In order to develop a isolation method of mycobacteria by tissue cultures, the isolation strain of mycobacterium bovis-M.bovis YL strain was inoculated in the tissue cultures of swine alveolar macrophages (SAM),Marc-145 and OCC, respectively．The results revealed that the M. bovis-YL strain was found growth well in the above mention tissue cultures at 3 day after inoculation．
The specimens collecting from the positive reaction cattle by PPD(Purified protein derivative) inoculating in slants (Lowenstein-Jensen medium,L-J M) and Marc-145 cell line , respectively, for isolating the M.bovis ．The results of the above test revealed that the M. bovis was isolated at 7 to 14 days after inoculating in Marc-145 cell lines﹐and isolated at 4 to 15 weeks after culturing in slants．Both methods of isolating rate were as same as 43.46%(33/76)．
|