Y. S. Lu', I. S. Tsai', M. H. Jong', P. P. Liu', Y. L. Lee', S. Y. Yang', C. IL Wang' and J. T. Wang'
Six strains of virus were isolated from brain, spleen, or intestine of pigs in 3 outbreak cases i. e. pigs with nervous symptom at Taoyung in Nov. 1975; pigs with perivascular lymphocytic cufing in brain at Peikung in Feb. 1976 and nursing piglets with severe diarrhea at Kaoh-sung in Aug. 1976. The isolates did not show any cytopathic effect (CPE) in primary pig kidney cultures but showed rounding changes with small plagues in the cultures after 3 passages. The results of physiochemical characters of the isolates indicated that they belonged to swine enterovirus. Morphological observation revealed that they caused type I CPE, however, serological tests, as compared with 10 kinds of standard antisera drived from Japan, all six isolates belonged to serotype J5 (WHO/FAO serotype 6). Sera collected from farms were subjected to neutralization tests, and 97.796 of them had antibody level of 1 :10 or higher.