| Summary |
The established hamster lung cell line, V79-379A could grow in a suspended state. The effect of changes in the conditions which control the growth of V79 cells in suspension were examined. The maximum cell yield was attained at 70rpm. The initial cell count, 1.0x105 /ml increased to 1.3x106 /ml on 4th day of culture with the higher cell growth rate (Ct/Co) than any other higher initial cell counts. Serum at a concentration of 4% was more suitable for cell culture than that at 8 % and 10%. Cells in suspended state could be passaged during different state, e.g. on 3rd or 4th day, with the same growth potential. Cells from the 3rd day suspended culture, though Kept at 4° C for 2 days, did not lose their Viability and could grow as well as those passaged immediately, but It was not true for the longer cultured cells.
The suspension culture of V79 cells was proved satisfactory for propagation of pseudorabies virus. The viral titer reached a maximum of 108 .6 TCID50 /ml. The viral yeild per cell from the suspension culture was higher in comparison with that from the monolayer cultures either in stationary or in rolling condition.
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