| Summary |
Babesia spp had been isolated from a cattle herd in Pintung area, then inoculated into young calf and the infected blood was obtained for antigen preparation. The antigen for complement-fixation (CF) test was prepared by distilled water extraction while antigen for capillary-tube agglutination (CA) test was prepared by sonication. CF antigen from the third to the fifth extraction had better antigenicity and did not have any anticomplementary activity. It's titer reached as high as I :8 while CA antigen showed only I : 2 against positive serum. By the comparison with positive sera of field cases, the accuracy of CF test was 100% (10/10) while that of CA test was 60% (6/10). It was proved that CF test is more sensitive than CA test by this study.
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