AHRI report No.40

Phylogenetic analysis of classical swine fever virus in Taiwan

1.Department of Hog Cholera,Animal Health Research Institute, Council of Agriculture

2.Department of Veterinary Medicine, National Taiwan University

3.Department of Medical Research, Mackay Memorial Hospital

Two envelope glycoprotein (E^rns and E2) regions of the classical swinefevervirus (CSFV)were amplified by RT-PCR and sequenced directly from 158 specimen scollected between 1989 and 2003 in Taiwan.Phylogeneticanalysis of the two regions revealed a similar tree topology and the E^rns region provided better discrimination than the E2 region.One hundred and fifteen isolatesout of the 158 isolates were clustered with in subgroup2.1(furtherclassifiedas2.1aand2.1b) and 2.2, which were considered to be likely of the introduced strains, whereas the remaining 43 isolates were clustered within subgroup 3.4 and were considered to be of the end emicstrains.The subgroup2.1a viruses were first detected in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in the early years, not being isolated after 1996. We have observed a dramatic switch in genotype from subgroup 3.4 to 2.1a. The subgroup 2.1a isolates are closely related to the Paderborn and Lao isolates, whereas 2.1b isolates have a close relationship to the Chinese Guangxi isolates. The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (Erns–E2) displayed better resolution than that based on the complete open reading frame.

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