Non-Woven-polyethylene-Fabric (NWPF) can provide high surface to volume ratio and three-dimensional structure for cells to grow. Appling cell culture bioreactor with NWPF carrier to scale up animal vaccine is beneficial for saving cost and increasing effiecient as well as for promoting the quality and competition of those products. Additionally, it could be avalible for the preliminary approach about propagation of high titer virus supernatant.