The HYT virus, strain FC-126, aft propagated in SF? GEE cultures, harvesti, and stored in liquid nitrogen, shown no kaiia, fungh or mycoplasma conaination. The sJ virus did not contaminate with any hemagglutinating virus. High level (10 doseslbixd) of the virus did not induce in chick incorlatal at one day of age any MD
clinical signs or MD grc€s lesions dun jig the 4-months experimental poriod. The seed virus could protat the chicks against challenge of MD virus isolatal from chickens in Taiwan, The virus meets the rjpixement quoted in “Gtde of Federal Regulations”
TitleQ,Chapterl,#113. 165asaMastersivirus,andisreyfortheusein prcduction of the vaccines.