| Summary |
Monoclonal antibody (mAb) 2E6 against \?2 capsid protein of infectious brusal disease virus (IBDV) or rabbit anti-IBDV antis erurn wasinitially used to capture the IBDV antigens in bursal tissue specimens followed by an enzyme linked intmunosorbent assay (sandwich ELISA) for the detection of ]BD infection. IBDV antigens in 16 from 24 bursal specimens could be detected. The ratio of aborbance value from IBDV-positive and -negative bursal tissues (RN) was 7.5 when the nAb was initially used in sandwich ELISA, while that was 6.9 when rabbit anti-IBDV antiserum was used. It suggests that the sandwich ELISA following the antigen captuure with both kinds of antibody could clearly differentiate th PN (IBDV-positive and negative bursal tissues). Moreover, the sandwich ELISA with nAb for the detection of IBDV with mAb for the detection of IBDV is better than that with antiserum.
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