| Department |
1.Taiwan Provincial Rsearch Institute for Animal Health, Taipei, Taiwan 251, R.O.C
2.Department of Veterinary Medicine, Animal Industry Research Institute TSC, Chunan, Miaoli, Taiwan 350, R.O.C
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| Summary |
Anaplasma marginale, and infectious agent, was successfully isolated from naturally infected cattle originating from a farm in northern Taiwan. Three serological tests, including complement-fixation test ( CF ), enzyme-linked immunosorbent assay ( ELISA ) and indirect fluorescent test ( IFA ) were developed to detect antibodies to A marginale in ruminants. A marginale organisms isolated from an infected calf were used as the CF, ELISA, and IFA antigens. There were no significant cross reactions between the antigens and the cattle hyperimmune sera produced by Bebesia bigemina, Theileria sergenti, and Eperythrozoon wenyoni. Comparative testiog among the 3 serological tests showed that the CF had the highest. sensitivity and the IFA the least. The ELISA, as compared to CF, was found to have relative agreement and sensitivity of94.4 and 89.1 %, respectively, whereas the relative agreement and sensitivity between the CF and IFA were 87.9 and 73.9 %, respectively. A serological survey was carried out to determine the prevalence of antibodies to A marginale in cattle and goats in Taiwan using CF, ELISA, and
IF A tests. The results indicate that the three tests had a high degree of agreement. Of the 915 cattle serum samples from 8 prefectures tested, 283 ( 30.9 % ) were positive. Col-lectively, all 8 prefectures had A marginale antibodies. Among these, Taipei prefecture had the highest prevalence ( 62.3 % ), followed by Hsinchu ( 47.5 % ), Taoyuen ( 43.2 % ), Miaoli ( 35.8 % ), Taichung ( 16 % ), Pingtung ( 12.5 %, ), Tainan ( 11.8 % ), and Taitung ( 2.7 % ). In goats, only two of the 4 prefectures submitting serum samples had A margi-nale antibodies with prevalence of 7 and 4 %, respectively.
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