A high performance liquid chrornatographic methcd for detrrraation of chloxamphenicol (CM) in prawn and eel fJs has ken developel. It could obtain good sexation and linear calibration curve of CM when being. 3 chromatographed on Spheri- I RP- 18 column and mobile phase consisting of 40% MDH-0. 1M CH(X)ONH4. Three sulfa dngs, 3 synthetic bactericcides and chiortetracycline did&t interfere the &tctiom The prc*edure involved double extraction with water and cleanup on a Sep-Pak Cl 8 cartridge. Recoveries of CM in prawn and eel feet were 87.3- 89.8% and 88.6-89.8%, respectively.