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Update Date: [2021-06-10]

Publication High Performance Liquid Chromatographic Determination of Chloramphenicol in Prawn and Eel Feeds

NO.: 26
Reportno.

AHRI report No.26

Topic

High Performance Liquid Chromatographic Determination of Chloramphenicol in Prawn and Eel Feeds

Department

Taiwan Animal Health Research Institute

Author S,Y. UnS.Y. Chiu
Summary

A high performance liquid chrornatographic methcd for detrrraation of chloxamphenicol (CM) in prawn and eel fJs has ken developel. It could obtain good sexation and linear calibration curve of CM when being. 3 chromatographed on Spheri- I RP- 18 column and mobile phase consisting of 40% MDH-0. 1M CH(X)ONH4. Three sulfa dngs, 3 synthetic bactericcides and chiortetracycline did&t interfere the &tctiom The prc*edure involved double extraction with water and cleanup on a Sep-Pak Cl 8 cartridge. Recoveries of CM in prawn and eel feet were 87.3- 89.8% and 88.6-89.8%, respectively.

Keyword

Chloxarnphenicol3iigh performance liquid chromatographic method7chlorteuacycline


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