| Department |
1.Taiwan Animal Health Research Institute. Taisui, Taipei, Taiwan, ROC
2.Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC
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| Summary |
Four primers according to the sequence of activator gene (apxIC) and structural gene (apxlA) of Apxl were synthesized and used for polymerase chain reaction (PCR) to amplify specific DNA fragment among 12 Ap serotypes. The results indicated that serotype 1, 5, 9, 10 and
11 had an expected length of amplified DNA fragment -with 3 primers. However, serotype 1, 9 and 11 had also obtained a particular length with the other primer according to the sequence of apxlCA gene. The sequences between 2994 and 3011 of apxIA of serotype 5, 10 detected by PCR were found different with those of serotype 1, 9 and 11. The PCR amplified 3.9 kb DNA fragments of apxICA fromoserotype 1, 5, 9, 10 and 11 were analyzed by digestion with 10 restriction enzymes. The HindlII , NaeI ' Bell , and Apa LI cutting sites of apxIA of serotype 1, 9 and 11 were different from those of serotype 5 and 10. The restriction map of the 3 kb DNA fragment of apxIA amplified from the local isolate of serotype 1, were identical to those reported previously. Chang WM and Lai SS. Comparison ofActinobacillus pleuropneumoniae RTX-toxin I gene (apxI) among serotypes.
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