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Update Date: [2021-06-10]

Publication Differentiation of Porcine Reproductive and Respiratory Syndrome Viruses Isolated in Taiwan by RT-PCR

NO.: 36
Reportno.

AHRI report No.36

Topic

Differentiation of Porcine Reproductive and Respiratory Syndrome Viruses Isolated in Taiwan by RT-PCR

Department

Animal Health Research bstinxt, 0’uncil of Agriculture. Eautive Yuan

Author Huang,tS*,,MH,Jong,W,J.TuandS,Y,Un
Summary

Fifty porcine reproductive and respiratory syndrome viruses (PRRSV), isolatal in 1992 to 21))) in Taiwan, were testi by reverse transcription and polymerase chain reaction (RT-PCR) with two set of primers, orfibandoif?, to differentiate the
Summary genotys. The orf lb primers were universal to all genotyjrs of PRRSV, the off? primers were only s&ific to the North America virus stains. Our result showI that all of virus isolates from Taiwan were amplified with both primer sets, suggesting that the viruses in Taiwan axe clcGely related to North America type in genotypes. 

Keyword

Porcine regcductive and respiratory syndrome virus, Reverse transcription and polymerase chain reaction


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