| Reportno. |
AHRI report No.32
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| Topic |
Viability of the Newcastle Disease Virus in the poultry carcass held at－20℃ and 4 ℃
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| Department |
1.Taiwan Animal Health Research Institut
2.Department of Vetrinary Mdicine, National Taiwan University 3,Couircil of Agrioriture, Exitive Yuan
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| Author |
Dth-Fa Lini, Hsiar*g-Jung Tsai2,, Chusig-Hwei Yao3, Yor*g-Siu Lul |
| Summary |
SPF thickens infeced with Newcastle disease via-us (ND V)were sacrificeri, and the organs (btast muscle, liver, and large intstine)were harvestl and sbrtl at 4 “C or -20 “C. Two months latx the frceen organs were defrceed and made into emulsions. The emulsions were inCCUtZI bib) 8-wk-old SPF chickens inixanuscularly C ll ) or via oral nuts. High mortality was found in IM incculation group (EO—95 %), while oral inoculation group had a 1Ci—20 % mortality. Typical lesions of Newcastle disease were found in incoilai SPF chickens. a storerl for five months, the froan organs were made Into emulsions and were inccula into 8-week-old SPF chickens via IM or in Jar ( 10 ) andin asal( IN) roats. High mortalitywas indwxdin IM U1I1]flfl inoculation group ( 8CJ) %), while 10 and N inoculation group had a 20-40 % mortality.
The emulsions made from organs fican for 8 months were inoculal into 10-week-old holler via IM or oral nuts. 1)1 incculationcaus 17-38 % mortality in inoculatl hollers, while oral inoculation inducal 0—i? % mortality. The emulsions of organs storal at 4 “C for? days were incculaterl into 12-week-ok! SPF chickens, and caused)—lC1) % mortalityby Th1 routand’?O—lCI) % mortalityby 10 and INrouts. Thus we goved that NOV could survive in the pultry carcass hele at -20 “C for at least 8 months andat4 “Cforatleast7days. Wealsogoveithatthesurvivalviniseswere still able to induce disease in chickens.
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| Keyword |
Newcastle disease virus, Frcn pc’ultcy meat, Chilled carcasses
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