H5N1 HPAI is still a continued epidemic in China and southeast Asia. The outbreaks also expanded into domestic or wild bird in more then 40 countries of Europ and Africa. Therefore, the purpose of this proposal will to establish a suitable primary-selection system to detect the antigen in the aboundance of surveillance samples. We will go on the AIV routine surveillance on domestic and wild bird in Taiwan and isolats' pathogenic and pathobilogical analysis. The results of this study will be given to a refer for our goverment in AI monitory and control strategy.