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In Aug, and Sep. of 1984, outbreaks of an acute disease of turkey wee found to occur in two fanns located in Taichung city and Tainan County respectively. Signs of the clinical disease of the turkey were characterized by depression, bloody droppings, and death. The pzimarv lesion involved an enlarged spleen shows a marble appearance, and the congestion or hemorrhage in intestines. Histopathological changes include severe congestion of the mucosa and loss of the tips of the viii in the intestines. And also the hvperplasia of the white pulp, marked proliferatoin of reticuloendothelial cell, and presence of’signet-ring” shaped nuclei containing acidophulic inclusion bidies.
Attempts to isolate the suspected causative virus in primary chicken. kidney cell and embryonic fibroblast cell cultrue have not been successful. With negative stain electron microscopy, the causative viurs was observed in the emulsion of the spleen. The virus was about SO rim in diameter across with icosahedral capsid composed of 252 capsomeres. This morphologic feature was consistent with that of an avian adenovirus. Conventional thin-section electron mcroscoopy also demonstrated the same viral particles in the splenic cells of the turkeys examined.
The emulsion of the spleen made from the turkeys examined was inoculated orally or intravenously into healthy turkeys and SPF chickens, and subsquently reinduced the clinical signs and the lesions in inoculated turkeys.
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