| Author |
H.J. Ko,'* Ching Chen, 1 C. C. Lu, 1 J. S. Lai, 1 Nae Wei Guo,'I. P. Chan,' C. M. Shau,2 S. N. Chen,3 J. T. Wang' and H. Y. Chang |
| Summary |
An inactivated bacterin consisting of Edwardsiella tarda, Aeromonas hydrophila and Flexibacter columnaris was developed. Immunization test using intramuscular injection (IM) and orally with fish meal were carried out in eels to evaluate the combined bacterins. Each eel in the IM group was injected with 1.0 mL of bacterin; the eels in the orally vaccination with meal group were given 6.0 mL per eel per day for two consecutive days. Two weeks and four weeks after immunization, respectively, for the IM group and the orally group, the eels were challenged with Edwardsiella tarda, Aeromonas hydrophila, Flexibacter columnaris and it's combined suspension of pathogens. respectively. The protection indexes (PI) obtained in the laboratory were log 101.7, log 101.3, log 101.0 and log 101.4 for the IM group and log 100.8, log 101.9, log 1010 and log 101.7 for the orally group, respectively. Using the same pathogens in the laboratory, the test were also conducted in Taishi branch of the TFRI, The PI of by using orally vaccination with meal were log 100.8, log 100.5, log 101.7 and log 100.9, respectively.
These experimental results showed that there are variation in the protection indexes after challenge. As a whole, however, the combined bacterin achieved good immunization results in eels against the Edwardsiellosis, red fin disease.and columnaris disease.
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| Keyword |
Eels, Edwardsiella tarda, Aeromonas hydrophila, Flexibacter columnaris, Combination bacterin
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