A dixea immunorerodicase ohnique for the identification of munoglobulincxDintaining cells in the seotion of bovine spleen is cbcrikei. This is applicable to fornalin fixoJ, paxaffin-enkakled maerial. It is kasI on an immunohistochenical method using reroxidase4akelled antibodies. Rabbit were immunized by purified bovine IgG2. The 1g02 fraction of rabbit anti-bovine 1g02 antiserum was onjugai with horseradish peroxidase. The pesence of immunoglobulin-cxntaining cells in bovine spleen seotions was revealed by staining the tissue-bound eroxidase-lakelhl antibody with diaminokenzidine then briefly countstainei wth Jiematoxylin.
The endogeneous Etroxidase was satisfactorily inbhibitl by beating the dewaxed seotion with a fresh 0,5% solution of hydrogen peroxidee in abiolute methanol for twenty minu.
The strflxe of iranunoglobulin-containing cells in bovine spleen seotions is clear, exations axe imanent, and retosective stadies of stored paraffin-erninided tissue axe po6sible.