An indirect enzyme-linked immuno-sorbent assay (ELISA), used a crude antigen, was developed to detect antibodies against goose parvovirus in goose serum samples. The best result was obtained by using 1: 100 diluted serum samples, 10 µg/ml rabbit anti-goose IgG, 1 :500 diluted peroxidase labelled goat anti-rabbit lgG, and by incubated the enzyme substrate for 30 min.
Evaluated by the ELISA, it was found that both the goose inoculated with the attenuated or inactivated goose parvovirus vaccine gave good antibody response, and the antibody titre could last· at least 8 to 16 weeks.
Fourty-six serum samples collected from Chungli area in 198 I were found negative in ELISA antibody; however, 3 out of 14 serum samples collected from Kaohsiung county in 1986 were found positive in ELISA antibody against goose parvovirus.
The ELISA was found to be more sensitive than agar gel precipitin test in detection of antibodies against goose par-vovirus.