| Reportno. |
AHRI report No.41
|
| Topic |
Detection of Waterfowl Parvovirus with Real-time Polymerase Chain Reaction
|
| Department |
Animal Health Research Institute, Council of Agriculture, Executive Yuan |
| Author |
Chen YP *, Lee MS, Lee SH |
| Summary |
The purpose of the study was to develop the assay of detection of warerfowl parvovirus with real-time polymerase chain reaction by proceeding with the Applied Biosystems 7500 Real-Time PCR System coupled with SYBR green. Waterfowl parvovirus was subdivided into goose parvovirus and Muscovy duck parvovirus. According to the conserved region of GPV and MDPV respectively, the specific primers were designed for real-time PCR. The constructed real-time PCR assay has a detection limit of 3 pg. The sensitivity was 100-fold higher than common PCR. The developed linear relationship between concentration of nucleic acid and cycle numbers of reaction was could be the base of the standard curve of nucleic acid, and further, it could be applied for quantifying the nucleic acid.
|
| Keyword |
Real-time polymerase chain reaction, Waterfowl parvovirus, Goose parvovirus, Muscovy duck parvovirus
|