| Summary |
Experiments were conducted to investigate the residue of apramycin (100 ppm) fed to finishing pigs for 30 days. Pigs were slaughtered after O to 5 days of withdrawal periods. Tissues and serum were sampled for apramycin determiation. Samples of muscle and tissues were extracted with methanol then concentrated and dried. The residues were dissolved by distilled water then tested by bioautography and microbiological cylinder plate methods. Serum and urine were added lN potassium hydroxide and 25 % trichloroacetic acid and through the Amberlite IRC-50(H•) Column, then eluted with 1 N ammonium solution. The eluent was concentrated and dried, dissolved by distilled water, then tested by bioautography and microbiological cylinder plate methods. The detection limit was 0.05 µglg(ml), average of recovery in tissue was 70.6 %, in serum was 55.7 %. On day O of withdrawal period, all tissues sampled were residued by apramycin, with the highest level in liver (1.8 µgig) and the lowest level in muscle (0.9 µgig). On day I of withdrawal period, no drug activity could be detected from muscle and stomach. From day 2, no drug activity could be detected from all tissues.
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