| Summary |
Ten ducks were highly immunized with purified swine IgO. The anti-swine IgO antisenim, 350m1 from the ten immunized ducks, was xsaltl out at 40% saturation in amrnonium sulfate and then chrornatgraphal through Sephacryl 5-3(I) column. The 75 and 85 serum globulins were colleztal and ahsorlM with Sepharc6e CL-4E conjugated by swine IgG. The duck anti-swine IgG anti&dy abiorlM in the affinity column was desorkalby0.1M glycine buffer, pH 2.5. Only one pxthpitation line of the desorked duck immunoglobulin was oberved when t1 in immuncelectrophoxesis anainst rabbit anti-duck whole sewn-i antiserum. This would suggest that the immunoglobulin isoland in this manner in the duck IgG &xording t the isolation of the puedpitation line in the immuncelectophoxesis analysis, Antiduck IgG antiserum was raised by inting a rabbit with the isola duck IgG. Only one prthpiition line of the anti-duck IgG antiserum when testl in iinmuncelectrophsesb against duck whole serum.
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