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Update Date: [2022-09-27]

Publication ELISA FOR DETECTING ANTIBODIES TO BRUCELLA ABORTUS IN BOVINE SERUM


Reportno.

AHRI report No.23

Topic

ELISA for Detecting Antibodies to Brucella Abortus in Bovin Serum

Department

Taiwan Provincial Research Institute for Animal Health.

Author Yi-Shing Wu 
Summary

An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Brnce/la abortus in bovine serum. The assay was standardized in terms of antigen coating condition, optimal antigen concen-tration, serum and conjugate dilutions. The most optimal conditions for antigen coating was that Ll'S antigen was diluted to lug/ml with carbonate buffer (pH 9.6) followed by coating at 4°C overnight with 0.1 ml of diluted an.tigen. The gelatin NET solution was most effective in blocking. The negative cutoff point was s.et at 0.40 while serum sample was diluted to 1: 100. 
The relative sensitivity and specificity of ELISA to both results of complement fixation test and tube agglutination test were 100% in 22 diagnostic positive sera. The c-0rrelation coefficients of ELISA to these two tests were 0.927 and 0.943.

Keyword

ELISA ;

 


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