According to "Feed Additive Compendium", the salinomycin can be used only on poultry feeds (50-70 ppm) to prevent coccidiosis. High-performance liquid chromatography (HPLC) methods using post-column derivation with vanillin were described for the determination of salinomycin. This project tries to compare with the system suitability among different methods. So that a proper analysis method is created in the lab to identify and prevent from abusing feed additives.