| Department |
1.Animal Health Research Institute, Council of Agriculture, Executive Yuan
2.Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Taipei, Taiwan.
3.Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan.
4.Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan. |
| Summary |
The detection of antibiotics residue is generally analyzed by the growth inhibition ring of sensitive microbial strains revealed on agar plate diffusion test. An integrated efficient detection system of initial screening detection and subsequent differential detection is performed in this research report. The initial screening detection adopts microbial gel coloring method (MGCM) with permitted commercial package on antibiotic residue detection over various animal products. The Premi®Test kit by Dutch DSM food company effectively covers 9 antibiotics classes with 34 drugs of routine usage including beta-lactams, tetracyclines, aminoglycosides, and sulfa drugs. Our detection result with Premi®Test kit revealed superior detection sensitivity on antibiotic residue classes as to be more sensitive than allowed maximal residue quantity of government regulation. Moreover, the subsequent differential detection adopts four plate test (FPT) with permitted microbial media and sensitive microbial strains on antibiotic residue detection over various animal products. Merck antibiotic test agars (MATA) are respectively applied on four plates with catalog numbers of [I] 10663 pH 6.0, [II] 105270 pH 7.2, [III] 10664 pH 8.0, and [IV] 10664 pH 8.0 in which plates [I, II, III] are inoculated with Bacillus subtilis ATCC 6633 and plate [IV] with Sarcina lutea ATCC 9341. Screening and differential detection among several routine antibiotics classes TCC 9341. Screening and differential detection among several routine antibiotics classes can be accomplished by respective sensitivity ranking pattern of antibiotic residue with FPT inhibitory ring sizes including beta-lactams [IV >I >III >II (or II> III)], tetracyclines [I >II >III >IV], aminoglycosides [III >II >I >IV], macrolides [IV >III>II >I].
In conclusion, the integrated detection system of initial screening detection and subsequent differential detection may be evidently efficient in fulfilling practical goal of antibiotic residue detection over various animal products. Initial screening detection of MACM may be established as voluntary pre-detection by food product suppliers. Subsequent differential detection of FPT may be established as surveillance detection as of integrated detection system by regulatory institutions. Methodology performance of best detection limit is effectively within allowed veterinary drug residue limit supervised by the Department of Health which in turn efficiently assures regulatory compliance on legal standards in allowed maximal antibiotics residue quantity.
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