| Summary |
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of Olaquindox (ODX) , Carbadox (CDX) , Furazolidone (FZD) , Sulfathiazole (STZ), Sulfamethazine (SMT) , Nalidixic Acid (NAA) , Morante! citrate (MRT) , Nitrofurazone (NFZ) , and Difurazone (DFZ) in pig feeds was developed. 5 gm of samples were extracted with acetonitrile and centrifuged. The repeated extract were combined and cleaned with Al20 3 (acidic) column. The elute was concentrated by rotatory evaporator to dryness, and dissolved in 1 ml of acetonitrile : 0.1 % phosphoric acid ( 4 : 6 ) for analysis by HPLC. The HPLC conditions were established on a Mightysil RP-18 GP 150X 4.6 mm ( 5 um) cica-Reagent column with acetonitrile / 0.1 % phosphoric acid as mobile phase and detected at UV 260 nm and UV 360 nm. The feeds samples were spiked with synthetic antimicrobial drug in 0.625, 2.5 and 10 ppm were analysed. With 80 % acetonitrile : 0.1 % phosphoric acid ( 25: 75 ) as mobile phase and detected at UV 360 nm. The average recoveries of CDX ' MRT ' FZD were 72.5 % , 95.3 % , 100.1 %. With 80 % acetonitrile : 0.1 % phosphoric acid ( 20 : 80 ) as mobile phase and detected at UV 260 nm. The average recoveries of STZ , SMT , NFZ , FZD were 66.2 % , 99.3 % , 82.1 % , 95.6 %. With 80 % acetonitrile : 0.1 % phosphoric acid ( 35 : 65 ) as mobile phase and detected at UV 360 nm. The average recovery ofDFZ was 94.7 %. Except NAA and ODX have a poor recovery rate, CDX, NFZ , MRT , FZD , SMT , STZ , DFZ all had a good linear result. Value ofR was about 1.
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